Assembly

DNA sequence data has become an indispensable tool for Molecular Biology & Evolutionary Biology. Study in these fields now require a genome sequence to work from. We call this a ‘Reference Sequence.’ We need to build a reference for each species. We do this by Genome Assembly. De novo Genome Assembly is the process of reconstructing the original DNA sequence from the fragment reads alone.

Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides () and tutorial () buttons below.
Lesson Slides Hands-on Recordings Input dataset Workflows
An Introduction to Genome Assembly
An introduction to get started in genome assembly and annotation
Assembly of metagenomic sequencing data
assembly metagenomics microgalaxy
Assembly of the mitochondrial genome from PacBio HiFi reads
Chloroplast genome assembly
plants nanopore jbrowse1
De Bruijn Graph Assembly
Decontamination of a genome assembly
Deeper look into Genome Assembly algorithms
ERGA post-assembly QC
plants animals genome assembly QC
Genome Assembly Quality Control
assembly quality control
Genome Assembly of MRSA from Oxford Nanopore MinION data (and optionally Illumina data)
nanopore assembly amr gmod jbrowse1 microgalaxy
Genome Assembly of a bacterial genome (MRSA) sequenced using Illumina MiSeq Data
illumina assembly microgalaxy
Genome assembly using PacBio data
assembly pacbio
Large genome assembly and polishing
assembly polishing nanopore plants
Making sense of a newly assembled genome
gmod prokaryote microgalaxy jbrowse1
Unicycler Assembly
prokaryote microgalaxy
Unicycler assembly of SARS-CoV-2 genome with preprocessing to remove human genome reads
covid19
Using the VGP workflows to assemble a vertebrate genome with HiFi and Hi-C data
pacbio eukaryote VGP
Vertebrate genome assembly using HiFi, Bionano and Hi-C data - Step by Step
pacbio eukaryote VGP

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoSimon Gladman avatar Simon GladmanAnton Nekrutenko avatar Anton Nekrutenkoorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoCristóbal Gallardo avatar Cristóbal Gallardo

Contributors

This material was contributed to by:

orcid logoAnna Syme avatar Anna Symeorcid logoSimon Gladman avatar Simon Gladmanorcid logoAlex Ostrovsky avatar Alex Ostrovskyorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoLaura Leroi avatar Laura LeroiBrandon Pickett avatar Brandon Pickettorcid logoAlexandre Cormier avatar Alexandre CormierAnton Nekrutenko avatar Anton NekrutenkoMarcella Sozzoni avatar Marcella Sozzoniorcid logoLinelle Abueg avatar Linelle Abuegorcid logoStéphanie Robin avatar Stéphanie RobinGiulio Formenti avatar Giulio Formentiorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoPolina Polunina avatar Polina Poluninaorcid logoHelena Rasche avatar Helena Rascheorcid logoBérénice Batut avatar Bérénice BatutBazante Sanders avatar Bazante SandersFabian Recktenwald avatar Fabian Recktenwaldorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoWolfgang Maier avatar Wolfgang Maierorcid logoTom Brown avatar Tom Brownorcid logoErwan Corre avatar Erwan Corre

Funding

These individuals or organisations provided funding support for the development of this resource

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Gallantries
This project (2020-1-NL01-KA203-064717) is funded with the support of the Erasmus+ programme of the European Union. Their funding has supported a large number of tutorials within the GTN across a wide array of topics.
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ABRomics
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Avans