Training material for all kinds of transcriptomics analysis.

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.


Before diving into this topic, we recommend you to have a look at:



Start here if you are new to RNA-Seq analysis in Galaxy

Lesson Slides Hands-on Recordings Input dataset Workflows Galaxy servers
Introduction to Transcriptomics
plain text
Reference-based RNA-Seq data analysis tutorial
  • De novo transcriptome reconstruction with RNA-Seq
  • End-to-End Analysis

    These tutorials take you from raw sequencing reads to pathway analysis

    Lesson Slides Hands-on Recordings Input dataset Workflows Galaxy servers
    1: RNA-Seq reads to counts tutorial
    2: RNA-seq counts to genes tutorial
    3: RNA-seq genes to pathways tutorial


    Tutorials covering data visualisation

    Lesson Slides Hands-on Recordings Input dataset Workflows Galaxy servers
    RNA Seq Counts to Viz in R tutorial
  • Visualization of RNA-Seq results with CummeRbund
    plain text tutorial
    Visualization of RNA-Seq results with heatmap2
    Visualization of RNA-Seq results with Volcano Plot
  • Visualization of RNA-Seq results with Volcano Plot in R tutorial

  • Other

    Assorted tutorials

    Lesson Slides Hands-on Recordings Input dataset Workflows Galaxy servers
    CLIP-Seq data analysis from pre-processing to motif detection
    Differential abundance testing of small RNAs
    GO Enrichment Analysis
    Network analysis with Heinz plain text tutorial
    RNA-RNA interactome data analysis
    RNA-Seq analysis with AskOmics Interactive Tool
    plain text tutorial
    Small Non-coding RNA Clustering using BlockClust
    Whole transcriptome analysis of Arabidopsis thaliana plain text tutorial
  • Galaxy instances

    You can use a public Galaxy instance which has been tested for the availability of the used tools. They are listed along with the tutorials above.

    You can also use the following Docker image for these tutorials:

    docker run -p 8080:80

    NOTE: Use the -d flag at the end of the command if you want to automatically download all the data-libraries into the container.

    It will launch a flavored Galaxy instance available on http://localhost:8080. This instance will contain all the tools and workflows to follow the tutorials in this topic. Login as admin with password password to access everything.

    Frequently Asked Questions

    Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.


    This material is maintained by:

    orcid logoAvatarBérénice BatutAvatarMaria DoyleAvatarFlorian Heyl

    For any question related to this topic and the content, you can contact them or visit our Gitter channel.


    This material was contributed to by:

    AvatarMaria DoyleAvatarBelinda PhipsonAvatarHarriet DashnowAvatarJovana MaksimovicAvatarAnna TrigosAvatarMatt RitchieAvatarShian SuAvatarCharity LawAvatarFlorian HeylAvatarDaniel Maticzkaorcid logoAvatarBérénice Batutorcid logoAvatarAnthony BretaudeauAvatarGildas Le Corguilléorcid logoAvatarErwan CorreAvatarXi LiuAvatarMallory FreebergAvatarIGC Bioinformatics UnitAvatarChao (Cico) ZhangAvatarAnton Nekrutenkoorcid logoAvatarFotis E. Psomopoulosorcid logoAvatarToby HodgesAvatarErasmus+ ProgrammeAvatarPavankumar VidemAvatarXavier GarnierAvatarAnne SiegelAvatarOlivier Dameronorcid logoAvatarMateo BoudetAvatarAndrea BagnacaniAvatarCristóbal Gallardoorcid logoAvatarBeatriz Serrano-Solanoorcid logoAvatarAnika ErxlebenAvatarMarkus WolfienAvatarMo HeydarianAvatarClemens Blankorcid logoAvatarNicola Soranzoorcid logoAvatarPeter van Heusdenorcid logoAvatarLucille Delisle