Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

Hélène Chiapello avatar Hélène Chiapelloorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoMaria Doyle avatar Maria DoyleAnton Nekrutenko avatar Anton Nekrutenkoorcid logoErwan Corre avatar Erwan Correorcid logoMateo Boudet avatar Mateo BoudetEkaterina Polkh avatar Ekaterina Polkhorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoTeresa Müller avatar Teresa Müllerorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoRomane LIBOUBAN avatar Romane LIBOUBANKenji Fujihara avatar Kenji Fujiharaorcid logoBérénice Batut avatar Bérénice Batutorcid logoFelicitas Kindel avatar Felicitas KindelGildas Le Corguillé avatar Gildas Le CorguilléBazante Sanders avatar Bazante SandersValentin Loux avatar Valentin LouxWilliam Durand avatar William Durandorcid logoBjörn Grüning avatar Björn GrüningJasper Ouwerkerk avatar Jasper OuwerkerkNiall Beard avatar Niall BeardTwishi Gulati avatar Twishi Gulatiorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoLaura Leroi avatar Laura Leroiorcid logoSimon Gladman avatar Simon Gladmanorcid logoHelena Rasche avatar Helena Rascheorcid logoAnika Erxleben avatar Anika ErxlebenGuillaume Gautreau avatar Guillaume Gautreauorcid logoAlexandre Cormier avatar Alexandre CormierTorsten Seemann avatar Torsten SeemannNathan Dunn avatar Nathan Dunnorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoNicola Soranzo avatar Nicola SoranzoEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoStéphanie Robin avatar Stéphanie RobinSimon Bray avatar Simon Brayorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoPaul Zierep avatar Paul Ziereporcid logoAnna Syme avatar Anna Syme

Funding

These individuals or organisations provided funding support for the development of this resource