Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

Frequently Asked Questions

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoAnthony Bretaudeau avatar Anthony BretaudeauEkaterina Polkh avatar Ekaterina Polkhorcid logoDeepti Varshney avatar Deepti VarshneyNathan Dunn avatar Nathan DunnHélène Chiapello avatar Hélène Chiapelloorcid logoPaul Zierep avatar Paul Ziereporcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoHelena Rasche avatar Helena RascheEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoMaria Doyle avatar Maria Doyleorcid logoTeresa Müller avatar Teresa Müllerorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoErwan Corre avatar Erwan Correorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoMateo Boudet avatar Mateo Boudetorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoTristan Reynolds avatar Tristan ReynoldsTwishi Gulati avatar Twishi Gulatiorcid logoJonathan Kreplak avatar Jonathan KreplakKenji Fujihara avatar Kenji FujiharaGuillaume Gautreau avatar Guillaume Gautreauorcid logoBérénice Batut avatar Bérénice Batutorcid logoBjörn Grüning avatar Björn GrüningGildas Le Corguillé avatar Gildas Le CorguilléTorsten Seemann avatar Torsten Seemannorcid logoNiklas Mayle avatar Niklas MayleBazante Sanders avatar Bazante Sandersorcid logoSimon Gladman avatar Simon GladmanJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoMiaomiao Zhou avatar Miaomiao ZhouValentin Loux avatar Valentin Louxorcid logoStéphanie Robin avatar Stéphanie RobinAnton Nekrutenko avatar Anton Nekrutenkoorcid logoSaim Momin avatar Saim MominNiall Beard avatar Niall BeardSimon Bray avatar Simon BrayWilliam Durand avatar William Durandorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoLaura Leroi avatar Laura Leroiorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoAnna Syme avatar Anna Symeorcid logoAlexandre Cormier avatar Alexandre Cormier

Funding

These individuals or organisations provided funding support for the development of this resource