Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

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Contributors

This material was contributed to by:

Jasper Ouwerkerk avatar Jasper OuwerkerkDeepti Varshney avatar Deepti Varshneyorcid logoHelena Rasche avatar Helena RascheEkaterina Polkh avatar Ekaterina PolkhWilliam Durand avatar William Durandorcid logoPaul Zierep avatar Paul ZierepAnton Nekrutenko avatar Anton NekrutenkoTeresa Müller avatar Teresa Müllerorcid logoBjörn Grüning avatar Björn GrüningBazante Sanders avatar Bazante Sandersorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoSaskia Hiltemann avatar Saskia HiltemannNathan Dunn avatar Nathan Dunnorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoFelicitas Kindel avatar Felicitas KindelGildas Le Corguillé avatar Gildas Le Corguilléorcid logoJonathan Kreplak avatar Jonathan KreplakTwishi Gulati avatar Twishi GulatiEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilNiall Beard avatar Niall BeardSimon Bray avatar Simon BrayTorsten Seemann avatar Torsten Seemannorcid logoSimon Gladman avatar Simon Gladmanorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoMateo Boudet avatar Mateo BoudetKenji Fujihara avatar Kenji Fujiharaorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoBérénice Batut avatar Bérénice Batutorcid logoAnna Syme avatar Anna Symeorcid logoMaria Doyle avatar Maria Doyleorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoLaura Leroi avatar Laura Leroiorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoErwan Corre avatar Erwan Correorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

Funding

These individuals or organisations provided funding support for the development of this resource