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Introduction to CRISPR screen analysis




last_modification Published: Mar 7, 2022
last_modification Last Updated: Mar 10, 2022

What is CRISPR?

.center[Overview of CRISPR knockout method]

.image-100[CRISPR method]

.pull-right[Adapted from Addgene]

Speaker Notes

What is a CRISPR screen?

.center[CRISPR screens enable high-throughput functional interrogation of a genome]

.image-100[CRISPR screen]

Speaker Notes

How are the cells for the screen set up?


.image-60[sgRNA library]




Speaker Notes

What is the difference between a negative and positive screen?

.image-100[Neg and pos screens]

.pull-right[Adapted from Addgene]

Speaker Notes

A workflow for CRISPR screen analysis

CRISPR workflow

Speaker Notes

Thank you!

This material is the result of a collaborative work. Thanks to the Galaxy Training Network and all the contributors! Galaxy Training Network Tutorial Content is licensed under Creative Commons Attribution 4.0 International License.