name: inverse layout: true class: center, middle, inverse <div class="my-header"><span> <a href="/training-material/topics/genome-annotation" title="Return to topic page" ><i class="fa fa-level-up" aria-hidden="true"></i></a> </span></div> <div class="my-footer"><span> <img src="/training-material/assets/images/GTN-60px.png" alt="Galaxy Training Network" style="height: 40px;"/> </span></div> --- # Genome annotation with Prokka --- ## Requirements Before diving into this slide deck, we recommend you to have a look at: - [](/training-material/topics) --- ### <i class="fa fa-question-circle" aria-hidden="true"></i><span class="visually-hidden">question</span> Questions - How to annotate a bacterial genome? - How to visualize annoted genomic features? --- ### <i class="fa fa-bullseye" aria-hidden="true"></i><span class="visually-hidden">objectives</span> Objectives - Load genome into Galaxy - Annotate genome with Prokka - View annotations in JBrowse --- <!-- To show speaker notes during a presentation: press c to clone the slides (opens a new window) on one window, press p to show speaker notes display the other window --> ### Overview - What is genome annotation - Tools for genome annotation - The tool "Prokka" --- ### Annotation  ??? - Annotations are biological or other features on a genome - e.g. ribosome binding site: a biological feature - e.g. the TTTTTT homopolymer: may or may not be biological but could be interesting --- ### Annotation  --- ### Prokka  --- ### <i class="fa fa-key" aria-hidden="true"></i><span class="visually-hidden">keypoints</span> Key points - Prokka is a useful tool to annotate a bacterial genome. - JBrowse can be used to inspect the annotation of a genome. --- ## Thank you! This material is the result of a collaborative work. Thanks the [Galaxy Training Network](https://wiki.galaxyproject.org/Teach/GTN) and all the contributors (Anna Syme, Torsten Seemann, Simon Gladman) ! <img src="/training-material/assets/images/GTN.png" alt="Galaxy Training Network" style="height: 100px;"/>