Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

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Contributors

This material was contributed to by:

orcid logoBérénice Batut avatar Bérénice Batutorcid logoMateo Boudet avatar Mateo Boudetorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoMaria Doyle avatar Maria Doyleorcid logoStéphanie Robin avatar Stéphanie RobinSimon Bray avatar Simon Brayorcid logoErwan Corre avatar Erwan Correorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoFelicitas Kindel avatar Felicitas KindelTorsten Seemann avatar Torsten SeemannGildas Le Corguillé avatar Gildas Le Corguilléorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoPaul Zierep avatar Paul ZierepEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoDelphine Lariviere avatar Delphine LariviereDeepti Varshney avatar Deepti VarshneyValentin Loux avatar Valentin Louxorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoAnna Syme avatar Anna SymeJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoLaura Leroi avatar Laura LeroiTeresa Müller avatar Teresa MüllerNathan Dunn avatar Nathan DunnKenji Fujihara avatar Kenji Fujiharaorcid logoSimon Gladman avatar Simon GladmanBazante Sanders avatar Bazante Sandersorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoHelena Rasche avatar Helena RascheNiall Beard avatar Niall BeardWilliam Durand avatar William DurandGuillaume Gautreau avatar Guillaume Gautreauorcid logoJonathan Kreplak avatar Jonathan KreplakTwishi Gulati avatar Twishi Gulatiorcid logoBjörn Grüning avatar Björn GrüningEkaterina Polkh avatar Ekaterina Polkhorcid logoSaskia Hiltemann avatar Saskia HiltemannAnton Nekrutenko avatar Anton Nekrutenkoorcid logoRomane LIBOUBAN avatar Romane LIBOUBANHélène Chiapello avatar Hélène Chiapello

Funding

These individuals or organisations provided funding support for the development of this resource