Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

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Contributors

This material was contributed to by:

orcid logoNicola Soranzo avatar Nicola Soranzoorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoAnthony Bretaudeau avatar Anthony BretaudeauNathan Dunn avatar Nathan DunnAnton Nekrutenko avatar Anton Nekrutenkoorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoAlexandre Cormier avatar Alexandre CormierJasper Ouwerkerk avatar Jasper OuwerkerkSimon Bray avatar Simon Brayorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoBjörn Grüning avatar Björn Grüningorcid logoTeresa Müller avatar Teresa Müllerorcid logoSimon Gladman avatar Simon Gladmanorcid logoLaura Leroi avatar Laura LeroiGuillaume Gautreau avatar Guillaume GautreauEkaterina Polkh avatar Ekaterina Polkhorcid logoPaul Zierep avatar Paul Ziereporcid logoBérénice Batut avatar Bérénice BatutTorsten Seemann avatar Torsten SeemannHélène Chiapello avatar Hélène Chiapelloorcid logoJonathan Kreplak avatar Jonathan KreplakKenji Fujihara avatar Kenji Fujiharaorcid logoErwan Corre avatar Erwan CorreGildas Le Corguillé avatar Gildas Le Corguilléorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoMateo Boudet avatar Mateo Boudetorcid logoHelena Rasche avatar Helena RascheNiall Beard avatar Niall BeardBazante Sanders avatar Bazante SandersValentin Loux avatar Valentin LouxTwishi Gulati avatar Twishi GulatiEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoAnna Syme avatar Anna SymeWilliam Durand avatar William Durandorcid logoMaria Doyle avatar Maria Doyleorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoCristóbal Gallardo avatar Cristóbal Gallardo

Funding

These individuals or organisations provided funding support for the development of this resource