Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

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Contributors

This material was contributed to by:

Niall Beard avatar Niall Beardorcid logoAlexandre Cormier avatar Alexandre CormierNathan Dunn avatar Nathan Dunnorcid logoBjörn Grüning avatar Björn Grüningorcid logoLaura Leroi avatar Laura LeroiTeresa Müller avatar Teresa MüllerAnton Nekrutenko avatar Anton NekrutenkoTwishi Gulati avatar Twishi Gulatiorcid logoMaria Doyle avatar Maria Doyleorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoAnna Syme avatar Anna Symeorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoJonathan Kreplak avatar Jonathan KreplakWilliam Durand avatar William Durandorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoSimon Gladman avatar Simon GladmanSimon Bray avatar Simon Brayorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoMateo Boudet avatar Mateo BoudetTorsten Seemann avatar Torsten Seemannorcid logoHelena Rasche avatar Helena RascheEkaterina Polkh avatar Ekaterina PolkhEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoDelphine Lariviere avatar Delphine LariviereDeepti Varshney avatar Deepti Varshneyorcid logoFelicitas Kindel avatar Felicitas KindelJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoBérénice Batut avatar Bérénice BatutBazante Sanders avatar Bazante Sandersorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoErwan Corre avatar Erwan Correorcid logoPaul Zierep avatar Paul ZierepGildas Le Corguillé avatar Gildas Le CorguilléKenji Fujihara avatar Kenji Fujihara

Funding

These individuals or organisations provided funding support for the development of this resource