Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

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Contributors

This material was contributed to by:

Twishi Gulati avatar Twishi Gulatiorcid logoStéphanie Robin avatar Stéphanie Robinorcid logoLaura Leroi avatar Laura LeroiDeepti Varshney avatar Deepti Varshneyorcid logoDelphine Lariviere avatar Delphine LariviereEsteban Perez-Wohlfeil avatar Esteban Perez-Wohlfeilorcid logoSimon Gladman avatar Simon GladmanNathan Dunn avatar Nathan Dunnorcid logoBjörn Grüning avatar Björn Grüningorcid logoErwan Corre avatar Erwan Correorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoAlexandre Cormier avatar Alexandre CormierBazante Sanders avatar Bazante Sandersorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoMateo Boudet avatar Mateo BoudetGildas Le Corguillé avatar Gildas Le CorguilléKenji Fujihara avatar Kenji FujiharaEkaterina Polkh avatar Ekaterina Polkhorcid logoBérénice Batut avatar Bérénice BatutTeresa Müller avatar Teresa MüllerNiall Beard avatar Niall BeardTorsten Seemann avatar Torsten Seemannorcid logoAnna Syme avatar Anna SymeAnton Nekrutenko avatar Anton Nekrutenkoorcid logoPaul Zierep avatar Paul ZierepJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoMaria Doyle avatar Maria Doyleorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoAnika Erxleben avatar Anika ErxlebenSimon Bray avatar Simon Brayorcid logoFelicitas Kindel avatar Felicitas KindelWilliam Durand avatar William Durandorcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoHelena Rasche avatar Helena Rasche

Funding

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