Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

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Contributors

This material was contributed to by:

orcid logoBjörn Grüning avatar Björn Grüningorcid logoHelena Rasche avatar Helena RascheTwishi Gulati avatar Twishi GulatiValentin Loux avatar Valentin Louxorcid logoStéphanie Robin avatar Stéphanie RobinEkaterina Polkh avatar Ekaterina Polkhorcid logoAlexandre Cormier avatar Alexandre CormierHélène Chiapello avatar Hélène Chiapelloorcid logoDelphine Lariviere avatar Delphine Lariviereorcid logoErwan Corre avatar Erwan Correorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoAnthony Bretaudeau avatar Anthony BretaudeauNiall Beard avatar Niall Beardorcid logoAnna Syme avatar Anna SymeKenji Fujihara avatar Kenji Fujiharaorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoBérénice Batut avatar Bérénice Batutorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoLaura Leroi avatar Laura LeroiWilliam Durand avatar William DurandSimon Bray avatar Simon Brayorcid logoAnika Erxleben avatar Anika ErxlebenJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoMateo Boudet avatar Mateo BoudetAnton Nekrutenko avatar Anton Nekrutenkoorcid logoMiaomiao Zhou avatar Miaomiao ZhouNathan Dunn avatar Nathan Dunnorcid logoJonathan Kreplak avatar Jonathan Kreplakorcid logoSimon Gladman avatar Simon GladmanBazante Sanders avatar Bazante Sandersorcid logoPaul Zierep avatar Paul ZierepGuillaume Gautreau avatar Guillaume Gautreauorcid logoTeresa Müller avatar Teresa MüllerEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilTorsten Seemann avatar Torsten Seemannorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoNicola Soranzo avatar Nicola SoranzoGildas Le Corguillé avatar Gildas Le Corguilléorcid logoMaria Doyle avatar Maria Doyle

Funding

These individuals or organisations provided funding support for the development of this resource