Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Bacterial Genome Annotation
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)
Essential genes detection with Transposon insertion sequencing

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
From small to large-scale genome comparison
Comparative gene analysis in unannotated genomes

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoAnna Syme avatar Anna SymeJasper Ouwerkerk avatar Jasper OuwerkerkEkaterina Polkh avatar Ekaterina Polkhorcid logoHelena Rasche avatar Helena Rascheorcid logoBjörn Grüning avatar Björn Grüningorcid logoTeresa Müller avatar Teresa Müllerorcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoSimon Gladman avatar Simon GladmanAnton Nekrutenko avatar Anton Nekrutenkoorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoAnika Erxleben avatar Anika Erxlebenorcid logoPaul Zierep avatar Paul Ziereporcid logoMiaomiao Zhou avatar Miaomiao Zhouorcid logoNicola Soranzo avatar Nicola SoranzoTorsten Seemann avatar Torsten Seemannorcid logoMateo Boudet avatar Mateo Boudetorcid logoDeepti Varshney avatar Deepti Varshneyorcid logoBérénice Batut avatar Bérénice BatutWilliam Durand avatar William Durandorcid logoErwan Corre avatar Erwan CorreBazante Sanders avatar Bazante SandersNathan Dunn avatar Nathan DunnSimon Bray avatar Simon Brayorcid logoFelicitas Kindel avatar Felicitas Kindelorcid logoAlexandre Cormier avatar Alexandre Cormierorcid logoStéphanie Robin avatar Stéphanie RobinEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilGuillaume Gautreau avatar Guillaume Gautreauorcid logoMaria Doyle avatar Maria DoyleKenji Fujihara avatar Kenji FujiharaHélène Chiapello avatar Hélène Chiapelloorcid logoJonathan Kreplak avatar Jonathan KreplakTwishi Gulati avatar Twishi GulatiValentin Loux avatar Valentin LouxGildas Le Corguillé avatar Gildas Le Corguilléorcid logoLaura Leroi avatar Laura Leroiorcid logoAnthony Bretaudeau avatar Anthony BretaudeauNiall Beard avatar Niall Beardorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoDelphine Lariviere avatar Delphine Lariviere

Funding

These individuals or organisations provided funding support for the development of this resource