Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

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Requirements

Before diving into this topic, we recommend you to have a look at:

Material

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.

Introduction

Start here if you are new to genome annotation in Galaxy.

Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation

Prokaryotes

Annotation of prokaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Essential genes detection with Transposon insertion sequencing
Bacterial Genome Annotation
Functional profiling of a bacterial genome with Eggsplorer
Genome annotation with Prokka
Identification of AMR genes in an assembled bacterial genome
Refining Genome Annotations with Apollo (prokaryotes)

Eukaryotes

Annotation of eukaryotic genomes.

Lesson Slides Hands-on Recordings Input dataset Workflows
Masking repeats with RepeatMasker
Genome annotation with Funannotate
Genome annotation with Maker (short)
Genome annotation with Maker
Functional annotation of protein sequences
Refining Genome Annotations with Apollo (eukaryotes)
Long non-coding RNAs (lncRNAs) annotation with FEELnc
CRISPR screen analysis
Comparison of two annotation tools - Helixer and Braker3
Genome annotation with Braker3
Genome annotation with Helixer

Other

Assorted Tutorials

Lesson Slides Hands-on Recordings Input dataset Workflows
Comparative gene analysis in unannotated genomes
From small to large-scale genome comparison
Pangenome analysis with Roary

Learning Pathways

Or have a look at one of our learning pathways involving this topic. Learning pathways are sets of tutorials curated for you by community experts to form a coherent set of lessons around a topic, building up knowledge as you go. We always recommend to follow the tutorials in the order they are listed in the pathway.

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Editorial Board

This material is reviewed by our Editorial Board:

orcid logoHelena Rasche avatar Helena Rascheorcid logoAnthony Bretaudeau avatar Anthony Bretaudeau

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Contributors

This material was contributed to by:

orcid logoTristan Reynolds avatar Tristan Reynoldsorcid logoDelphine Lariviere avatar Delphine LariviereTorsten Seemann avatar Torsten Seemannorcid logoSolenne Correard avatar Solenne CorreardKenji Fujihara avatar Kenji Fujiharaorcid logoTeresa Müller avatar Teresa Müllerorcid logoAnna Syme avatar Anna Symeorcid logoDeepti Varshney avatar Deepti VarshneyNathan Dunn avatar Nathan DunnDonny Vrins avatar Donny VrinsTwishi Gulati avatar Twishi Gulatiorcid logoNiklas Mayle avatar Niklas MayleJasper Ouwerkerk avatar Jasper Ouwerkerkorcid logoBérénice Batut avatar Bérénice Batutorcid logoSaskia Hiltemann avatar Saskia Hiltemannorcid logoJonathan Kreplak avatar Jonathan KreplakAnton Nekrutenko avatar Anton Nekrutenkoorcid logoMaria Doyle avatar Maria Doyleorcid logoSaim Momin avatar Saim Mominorcid logoAnthony Bretaudeau avatar Anthony Bretaudeauorcid logoAnika Erxleben avatar Anika ErxlebenWilliam Durand avatar William Durandorcid logoPaul Zierep avatar Paul ZierepEkaterina Polkh avatar Ekaterina PolkhBazante Sanders avatar Bazante Sandersorcid logoCristóbal Gallardo avatar Cristóbal Gallardoorcid logoMateo Boudet avatar Mateo Boudetorcid logoHelena Rasche avatar Helena Rascheorcid logoNicola Soranzo avatar Nicola Soranzoorcid logoFelicitas Kindel avatar Felicitas KindelGuillaume Gautreau avatar Guillaume Gautreauorcid logoSimon Gladman avatar Simon Gladmanorcid logoLaura Leroi avatar Laura Leroiorcid logoAlexandre Cormier avatar Alexandre CormierNiall Beard avatar Niall BeardSimon Bray avatar Simon Brayorcid logoVesselin Baev avatar Vesselin BaevGildas Le Corguillé avatar Gildas Le Corguilléorcid logoRomane LIBOUBAN avatar Romane LIBOUBANorcid logoMiaomiao Zhou avatar Miaomiao ZhouHélène Chiapello avatar Hélène Chiapelloorcid logoBjörn Grüning avatar Björn Grüningorcid logoErwan Corre avatar Erwan CorreEsteban Perez-Wohlfeil avatar Esteban Perez-WohlfeilValentin Loux avatar Valentin Louxorcid logoStéphanie Robin avatar Stéphanie Robin

Funding

These individuals or organisations provided funding support for the development of this resource