When I get a warning for base per sequence content, what should I do?

So far it does not mean that your data is bad. Your protocol or your data might have a bias that you normally expect. Check first the following things:

• Adapter content (maybe some adapters are still in your data)
• Kmer content/Over represented sequences (this would indicate a contamination or a protocol/sequence bias)
• Per base quality plot. If the overall quality is not good, then probably the sequencing was poorly performed.
• Read about your protocol, e.g., ChIP-Seq and ATAC-Seq typically have a nucleotide bias. For example this article about ATAC-Seq.

Still have questions?

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{% snippet  topics/sequence-analysis/tutorials/quality-control/faqs/perbase_warning.md %}

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