Tool Dependencies and Containers

</span></div>

</span></div>

---

# Tool Dependencies and Containers

<div class="contributors-line">
		
	
<ul class="text-list">
			
			<li>
				
  <a href="/training-material/hall-of-fame/jmchilton/" class="contributor-badge contributor-jmchilton"><img src="/training-material/assets/images/orcid.png" alt="orcid logo" width="36" height="36"/><img src="https://avatars.githubusercontent.com/jmchilton?s=36" alt="John Chilton avatar" width="36" class="avatar" />
    John Chilton</a>
  
			<li>
				
  <a href="/training-material/hall-of-fame/bgruening/" class="contributor-badge contributor-bgruening"><img src="/training-material/assets/images/orcid.png" alt="orcid logo" width="36" height="36"/><img src="https://avatars.githubusercontent.com/bgruening?s=36" alt="Björn Grüning avatar" width="36" class="avatar" />
    Björn Grüning</a>
  </li>
</ul>

</div>

<div class="footnote" style="bottom: 8em;">
  <i class="far fa-calendar" aria-hidden="true"></i><span class="visually-hidden">last_modification</span> Updated:   
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???
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Useful when presenting.

---

## Requirements

Before diving into this slide deck, we recommend you to have a look at:

- [Development in Galaxy](/training-material/topics/dev)
    - Tool development and integration into Galaxy: [<i class="fab fa-slideshare" aria-hidden="true"></i><span class="visually-hidden">slides</span> slides](/training-material/topics/dev/tutorials/tool-integration/slides.html) - [<i class="fas fa-laptop" aria-hidden="true"></i><span class="visually-hidden">tutorial</span> hands-on](http://planemo.readthedocs.io/en/latest/writing_standalone.html)

---

### <i class="far fa-question-circle" aria-hidden="true"></i><span class="visually-hidden">question</span> Questions

- What are the advantages of running my Galaxy tool inside of a container?

- How does Galaxy find a container to run my tool in?

- What are BioContainers and how are they related to Galaxy?

---

### <i class="fas fa-bullseye" aria-hidden="true"></i><span class="visually-hidden">objectives</span> Objectives

- Explore the differences between containerizing Galaxy and tool execution.

- Discuss the advantages of containerizing tools.

- Learn to build best practice tools ready to be containerized.

---

## Planemo

These slides mirror the section on "Dependencies and Docker" in the [Planemo Documentation](https://planemo.readthedocs.io/en/latest/writing_advanced.html#dependencies-and-docker).

---

## Generic Containers are Good Slide

.image-75[![A cartoon comparing containers vs VMs. On the left the VMs are presented as a layer of server, host os, and hypervisor (type 2) followed by several tall pillars of guest OS, bin/libs and finally their respective apps. Everything is siloed. On the right is containers where the layers are server, host os, and docker engine. Above those are very short pillars of each different app, and when duplicate apps are run, the bin/libs are shown as shared. Text reads: containers are isolated but share OS and where appropriate, bins/libraries.](../../images/containers.png)]

* Isolation and Security\*
* Reproducibility
* Flexibility\*

???

---

## Galaxy in Containers?

.image-75[![Screenshot of docker-galaxy-stable readme showing the scheme with a lot of different galaxy container flavours shown as layers atop one another.](../../images/docker-galaxy-stable-github.png)]

???

When people think Galaxy and containers I think this project comes to mind.
Three years ago Björn created this docker-galaxy-stable project and it has
been wildly successful. It has proven to be a useful toolkit for advanced
deployment options, an extremely portable way to bring Galaxy to new domains,
and a brilliant tool for training users.

https://github.com/bgruening/docker-galaxy-stable/commit/27ef7966508958dfec9ce35ff1c5f076ffccf80f

Also mention Galaxy KickStart as a way to run Galaxy itself in a container.

---

## Containerizing Galaxy vs Tools

We are going to discuss containerizing tool execution instead - executing just jobs in containers.

Containers for the particular job's tool.

However you deploy Galaxy, including in a container, tool execution can still be containerized.

???

---

### Containerizing Tools is Still Important

* Isolated tool execution.
* Isolate file system access.
* Added layer of security.
* Increased re-computability.
* New deployment options - Kubernetes, Mesos Chronos, AWS Batch, etc.

---

### Containerizing Tool Execution

Decomposes to basic problems:

* <i class="fa fa-square-o" aria-hidden="true"></i> Instruct Galaxy where to find a container for the tool.
* <i class="fa fa-square-o" aria-hidden="true"></i> Instruct Galaxy to run the tool in a container.

---

### Configuring Galaxy to Use Containers

Just configure the destination. For instance, transform the cluster destination:

```
<destination id="short_fast" runner="slurm">
    <param id="nativeSpecification">--time=00:05:00 --nodes=1</param>
</destination>
```

as follows:

```
<destination id="short_fast" runner="slurm">
    <param id="nativeSpecification">--time=00:05:00 --nodes=1</param>
    <param id="docker_enabled">true</parma>
    <param id="docker_sudo">false</param>
</destination>
```

That is it!

For development, the Planemo flag `--docker` does this for `test`, `serve`, and related commands.

---

### Containerizing Tool Execution

#### Decomposes to basic problems

* <i class="fa fa-square-o" aria-hidden="true"></i> Instruct Galaxy where to find a container for the tool.
* <i class="fa fa-check-square-o" aria-hidden="true"></i> Instruct Galaxy to run the tool in a container.

---

## Explicit Container Dependencies

Returning to the seqtk example, lets change the `requirements` from:

```
<requirements>
    <requirement type="package" version="1.2">seqtk</requirement>
</requirements>
```

into

```
<requirements>
    <container type="docker">quay.io/biocontainers/seqtk:1.2--1</container>
    <requirement type="package" version="1.2">seqtk</requirement>
</requirements>
```

Now run Planemo `test` and `serve` with the `--docker` flag and as a tool developer you are done!

---

### Containerizing Tool Execution

#### Decomposes to basic problems

* <i class="fa fa-check-square-o" aria-hidden="true"></i> Instruct Galaxy where to find a container for the tool.
* <i class="fa fa-check-square-o" aria-hidden="true"></i> Instruct Galaxy to run the tool in a container.

We're done... right?

???

---

## The Problems with Making Docker Explicit

* Setting up a `Dockerfile` and publishing a Docker image more process for the tool even though the dependencies have already been completely defined.
* An arbitrary Docker image is a blackbox and there is no guarantee Galaxy will execute the same binaries as the Conda requirements.

---

## To Put it Another Way

This:

```
<requirements>
    <requirement type="package" version="1.2">seqtk</requirement>
</requirements>
```

should have been sufficient!

And the good news is that now it is (mostly)!

---

### .image-10[![biocontainers logo](../../images/biocontainers.png)] BioContainers - The Magic

Galaxy can now automatically find or build containers for best practice tools.

Planemo will check if such a container has been published with the `--biocontainers` flag to `planemo lint`.

```
$ planemo lint --biocontainers seqtk_seq.xml
...
Applying linter biocontainer_registered... CHECK
.. INFO: BioContainer best-practice container found [quay.io/biocontainers/seqtk:1.2--1].
```

???

But we didn't do anything - where did this magic seqtk container come from.

---

## The Mystery quay.io/biocontainers/seqtk Container

![screenshot of quay.io showing the repository tags for the seqtk repo](../../images/quayioseqtk.png)

???

I swear Planemo did not just create that with `tool_init` or `lint`, maybe we should try using it...

---

### .image-05[![biocontainers logo](../../images/biocontainers.png)] BioContainers - Using the Container

Run Planemo `test` or `serve` with `--biocontainers` to try mystery container.

```
$ planemo test --biocontainers seqtk_seq.xml
...
[galaxy.tools.actions] Setup for job Job[unflushed,tool_id=seqtk_seq] complete, ready to flush (20.380 ms)
[galaxy.tools.actions] Flushed transaction for job Job[id=2,tool_id=seqtk_seq] (15.191 ms)
...
[galaxy.tools.deps.containers] Checking with container resolver [ExplicitDockerContainerResolver[]] found description [None]
[galaxy.tools.deps.containers] Checking with container resolver [CachedMulledDockerContainerResolver[namespace=None]] found description [None]
[galaxy.tools.deps.containers] Checking with container resolver [MulledDockerContainerResolver[namespace=biocontainers]] found description [ContainerDescription[identifier=quay.io/biocontainers/seqtk:1.2--1,type=docker]]
[galaxy.jobs.command_factory] Built script [/tmp/tmpw8_UQm/job_working_directory/000/2/tool_script.sh] for tool command [seqtk seq -a '/tmp/tmpw8_UQm/files/000/dataset_1.dat' > '/tmp/tmpw8_UQm/files/000/dataset_2.dat']
...
ok

----------------------------------------------------------------------
XML: /private/tmp/tmpw8_UQm/xunit.xml
----------------------------------------------------------------------
Ran 1 test in 11.926s
OK
```
]

The important line here is - `Checking with container resolver [MulledDockerContainerResolver[namespace=biocontainers]] found description [ContainerDescription[identifier=quay.io/biocontainers/seqtk:1.2--1,type=docker]]`.

---

### .image-05[![biocontainers logos](../../images/biocontainers.png)] BioContainers - The Community

![screenshot of biocontainers github page](../../images/biocontainers_org.png)

???

BioContainers is a big community of informatics minded individuals trying to work
together on building container and Docker based solutions for problems like ours.
Previously the project was called BioDocker.

We are exploiting and contributing to one aspect of BioContainers, in particular
BioConda for Containers.

---

### .image-10[![biocontainers logo](../../images/biocontainers.png)] BioContainers - Bioconda for Containers

All Bioconda packages are built into minimal containers.

This setup allows the same binaries to be used within the container or on traditional/HPC resources.
Without any extra work by tool authors, Galaxy can automatically find or build “the correct” container for a best-practice tool.

Over 4,000 containers already published.

---

### .image-05[![biocontainers logo](../../images/biocontainers.png)] BioContainers - Lots of Containers

![screenshot of biocontainers on quay.io with lots of containers](../../images/quay_list.png)

???

TODO: Zoom in on the image a bit more ideally.

---

### .image-05[![biocontainers logo](../../images/biocontainers.png)] BioContainers - On Demand Creation

Galaxy can be configured to attempt to build containers on demand for containers that haven't
been published to the BioContainers namespace on quay.io.

These containers will have same names as would be published to quay.io (e.g. `quay.io/biocontainers/seqtk:1.2--0`).

---

### Containerizing Tool Execution

#### Decomposes to basic problems

* <i class="fa fa-check-square-o" aria-hidden="true"></i><i class="fa fa-check-square-o" aria-hidden="true"></i> Instruct Galaxy where to find a container for the tool.
* <i class="fa fa-check-square-o" aria-hidden="true"></i> Instruct Galaxy to run the tool in a container.

We're done this time now right?

???

---

## Revisiting Galaxy Requirements

![schematic of a galaxy server with dependency resolution via requirement tags at the top. On the left is the tool box with a number of xml files listed like seqtk_seq and seqtk_subseq. On the right is applications & libraries showing only a few tools like seqtk, all of the 3 multipoe subtools were collapsed](../../images/galaxy_instance.png)

Many tools have multiple requirements, these need containers also!

???

---

## An Example

The BWA tool is an example of one such tool that has multiple requirements - because samtools is used to sort BAMs after mapping. The Planemo `conda_testing` project is distributed with a simple tool to simulate this containing both `bwa` and `samtools` requirements.

.reduce90[
```
$ planemo project_init --template=conda_testing conda_testing
$ cd conda_testing/
$ grep -r require bwa_and_samtools.xml
bwa_and_samtools.xml:    <requirements>
bwa_and_samtools.xml:        <requirement type="package" version="0.7.15">bwa</requirement>
bwa_and_samtools.xml:        <requirement type="package" version="1.3.1">samtools</requirement>
bwa_and_samtools.xml:    </requirements>
```
]

---

## Container Hashing

Galaxy finds containers based on the names and versions of requirements, so far we have seen
the hash for single requirements is just the name and the version.

If multiple requirements are present, the hash looks more like a hash. For bwa @ 0.7.15 and samtools @ 1.3.1,
Galaxy will look for a container called:

.reduce50[`quay.io/biocontainers/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:03dc1d2818d9de56938078b8b78b82d967c1f820-0`]

---

## Galaxy Terminology - Mulled

To *mull* is to create an environment (either in the Conda sense or globally
inside a container) for one or more Conda packages. The result of this is a
*mulled* environment.

Fixed naming schemes for the resulting environments ensure that different tools
with the same set of requirements can reuse a previously created environment or
container.

If more than one package is included in the resulting environment, a complicated
hash is used as this name.

---

## Mulled Hashing

Back to the example of bwa at version 0.7.15 and samtools at version 1.3.1,
we said the resulting container will be

.reduce50[`quay.io/biocontainers/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:03dc1d2818d9de56938078b8b78b82d967c1f820-0`]

This can be broken into these parts:

```
quay.io/ <namespace> /mulled-v2-<package_hash>:<version_hash>-<build>
```

???

Explain the different parts.

---

## Exploring Mulled Hashes - by Evgeny Anatskiy

![Screenshot of biocontainers page showing an information page for multi-package containers](../../images/multi-package-containers.png)

???

---

## The Planemo `mull` command (1 / 2)

While Galaxy can be configured to auto-build BioContainers as they are needed, the Planemo `mull` command
can be used to manually build them for your local Docker host.

---

## The `mull` command (2 / 2)

```
$ planemo mull bwa_and_samtools.xml
/Users/john/.planemo/involucro -v=3 -f /Users/john/workspace/planemo/.venv/lib/python2.7/site-packages/galaxy_lib-17.9.0-py2.7.egg/galaxy/tools/deps/mulled/invfile.lua -set CHANNELS='iuc,bioconda,r,defaults,conda-forge' -set TEST='true' -set TARGETS='samtools=1.3.1,bwa=0.7.15' -set REPO='quay.io/biocontainers/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:03dc1d2818d9de56938078b8b78b82d967c1f820' -set BINDS='build/dist:/usr/local/' -set PREINSTALL='conda install --quiet --yes conda=4.3' build
/Users/john/.planemo/involucro -v=3 -f /Users/john/workspace/planemo/.venv/lib/python2.7/site-packages/galaxy_lib-17.9.0-py2.7.egg/galaxy/tools/deps/mulled/invfile.lua -set CHANNELS='iuc,bioconda,r,defaults,conda-forge' -set TEST='true' -set TARGETS='samtools=1.3.1,bwa=0.7.15' -set REPO='quay.io/biocontainers/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:03dc1d2818d9de56938078b8b78b82d967c1f820' -set BINDS='build/dist:/usr/local/' -set PREINSTALL='conda install --quiet --yes conda=4.3' build
DEBU Run file [/Users/john/workspace/planemo/.venv/lib/python2.7/site-packages/galaxy_lib-17.9.0-py2.7.egg/galaxy/tools/deps/mulled/invfile.lua]
STEP Run image [continuumio/miniconda:latest] with command [[rm -rf /data/dist]]
DEBU Creating container [step-730a02d79e]
DEBU Created container [5e4b5f83c455 step-730a02d79e], starting it
DEBU Container [5e4b5f83c455 step-730a02d79e] started, waiting for completion
DEBU Container [5e4b5f83c455 step-730a02d79e] completed with exit code [0] as expected
DEBU Container [5e4b5f83c455 step-730a02d79e] removed
STEP Run image [continuumio/miniconda:latest] with command [[/bin/sh -c conda install --quiet --yes conda=4.3 && conda install  -c iuc -c bioconda -c r -c defaults -c conda-forge  samtools=1.3.1 bwa=0.7.15 -p /usr/local --copy --yes --quiet]]
DEBU Creating container [step-e95bf001c8]
DEBU Created container [72b9ca0e56f8 step-e95bf001c8], starting it
DEBU Container [72b9ca0e56f8 step-e95bf001c8] started, waiting for completion
SOUT Fetching package metadata .........
SOUT Solving package specifications: .
SOUT
SOUT Package plan for installation in environment /opt/conda:
SOUT
SOUT The following packages will be UPDATED:
SOUT
SOUT conda: 4.3.11-py27_0 --> 4.3.22-py27_0
SOUT
SOUT Fetching package metadata .................
SOUT Solving package specifications: .
...
SOUT
DEBU Container [72b9ca0e56f8 step-e95bf001c8] completed with exit code [0] as expected
DEBU Container [72b9ca0e56f8 step-e95bf001c8] removed
STEP Wrap [build/dist] as [quay.io/biocontainers/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:03dc1d2818d9de56938078b8b78b82d967c1f820-0]
DEBU Creating container [step-6f1c176372]
DEBU Packing succeeded
```

This built `quay.io/biocontainers/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:03dc1d2818d9de56938078b8b78b82d967c1f820-0`!

???

---

## Testing locally `mull`ed containers

```
$ planemo test --galaxy_branch dev --biocontainers bwa_and_samtools.xml
...
[galaxy.tools.actions] Handled output named output_2 for tool bwa_and_samtools (17.443 ms)
[galaxy.tools.actions] Added output datasets to history (12.935 ms)
[galaxy.tools.actions] Verified access to datasets for Job[unflushed,tool_id=bwa_and_samtools] (0.021 ms)
[galaxy.tools.actions] Setup for job Job[unflushed,tool_id=bwa_and_samtools] complete, ready to flush (5.755 ms)
[galaxy.tools.actions] Flushed transaction for job Job[id=1,tool_id=bwa_and_samtools] (19.582 ms)
[galaxy.jobs.handler] (1) Job dispatched
[galaxy.tools.deps] Using dependency bwa version 0.7.15 of type conda
[galaxy.tools.deps] Using dependency samtools version 1.3.1 of type conda
[galaxy.tools.deps] Using dependency bwa version 0.7.15 of type conda
[galaxy.tools.deps] Using dependency samtools version 1.3.1 of type conda
[galaxy.tools.deps.containers] Checking with container resolver [ExplicitContainerResolver[]] found description [None]
[galaxy.tools.deps.containers] Checking with container resolver [CachedMulledContainerResolver[namespace=None]] found description [ContainerDescription[identifier=quay.io/ biocontainers/mulled-v1-01afc412d1f216348d85970ce5f88c984aa443f3:latest,type=docker]]
[galaxy.jobs.command_factory] Built script [/tmp/tmpQs0gyp/job_working_directory/000/1/tool_script.sh] for tool command [bwa > /tmp/tmpQs0gyp/files/000/dataset_1.dat 2>&1 ; samtools > /tmp/tmpQs0gyp/files/000/dataset_2.dat 2>&1]
[galaxy.tools.deps] Using dependency samtools version None of type conda
[galaxy.tools.deps] Using dependency samtools version None of type conda
ok

----------------------------------------------------------------------
XML: /private/tmp/tmpQs0gyp/xunit.xml
----------------------------------------------------------------------
Ran 1 test in 7.553s

OK
[test_driver] Shutting down
[test_driver] Shutting down embedded galaxy web server
[test_driver] Embedded web server galaxy stopped
[test_driver] Stopping application galaxy
...
[galaxy.jobs.handler] job handler stop queue stopped
Testing complete. HTML report is in "/home/planemo/workspace/planemo/tool_test_output.html".
All 1 test(s) executed passed.
bwa_and_samtools[0]: passed
```

---

<hands-on-title>Hands-on</hands-on-title>

![cartoon of two people jumping](../../images/exercise.png)

---

<hands-on-title>Hands-on</hands-on-title>

#### The Goal

- Use `--biocontainers` to build a container on-demand for a test tool.

---

<hands-on-title>Hands-on</hands-on-title>

#### Steps

- Run through the bwa_and_samtools.xml test tool and verify container creation.

```
$ planemo project_init --project_template=conda_testing conda_testing
$ cd conda_testing/
$ planemo mull bwa_and_samtools.xml
$ docker images # verify the container was built
$ # use docker run to verify the container has samtools and bwa.
$ planemo test --biocontainers bwa_and_samtools.xml
```

---

### Publishing Multi-Package Containers

![screenshot of a mulled biocontainer in quay.io. Nothing useful is depicted.](../../images/multi-package-repo.png)

???

We are currently monitoring tools-iuc, tools-devteam, and a half a dozen other repositories
daily for package combinations to publish.

---

### Publishing **Your** Multi-Package Containers

![Screenshot of the text editor in github, editing monitor.sh in galaxyproject/planemo-monitor](../../images/planemo-monitor.png)

---
### <i class="fas fa-key" aria-hidden="true"></i><span class="visually-hidden">keypoints</span> Key points

- It is becoming easier, more advantageous, and more common for Galaxy admins to run all tools within their own container.

- You can explicitly define a container for your tool - but it is easier and more reproducible to let Galaxy find or build one using your tool's best practice requirements.

- The Galaxy community will infrastructure to automatically build and/or publish containers for your tool as long as it defines best practice Conda dependencies.

- Planemo makes it easy to test your tool inside of containers.

---

## Thank You!

This material is the result of a collaborative work. Thanks to the [Galaxy Training Network](https://training.galaxyproject.org) and all the contributors!

<div class="contributors-line">
		
<table class="contributions">
	
	<tr>
		<td><abbr title="These people wrote the bulk of the tutorial, they may have done the analysis, built the workflow, and wrote the text themselves.">Author(s)</abbr></td>
		<td>
			
  <a href="/training-material/hall-of-fame/jmchilton/" class="contributor-badge contributor-jmchilton"><img src="/training-material/assets/images/orcid.png" alt="orcid logo" width="36" height="36"/><img src="https://avatars.githubusercontent.com/jmchilton?s=36" alt="John Chilton avatar" width="36" class="avatar" />
    John Chilton</a>
  
  <a href="/training-material/hall-of-fame/bgruening/" class="contributor-badge contributor-bgruening"><img src="/training-material/assets/images/orcid.png" alt="orcid logo" width="36" height="36"/><img src="https://avatars.githubusercontent.com/bgruening?s=36" alt="Björn Grüning avatar" width="36" class="avatar" />
    Björn Grüning</a>
  
		</td>
	</tr>

<tr class="reviewers">
		<td><abbr title="These people reviewed this material for accuracy and correctness">Reviewers</abbr></td>
		<td>
			
  <a href="/training-material/hall-of-fame/nsoranzo/" class="contributor-badge contributor-nsoranzo">
    <img src="https://avatars.githubusercontent.com/nsoranzo?s=36" alt="Nicola Soranzo avatar" width="36" class="avatar" />
  </a>
  
  <a href="/training-material/hall-of-fame/hexylena/" class="contributor-badge contributor-hexylena">
    <img src="https://avatars.githubusercontent.com/hexylena?s=36" alt="Helena Rasche avatar" width="36" class="avatar" />
  </a>
  
  <a href="/training-material/hall-of-fame/bebatut/" class="contributor-badge contributor-bebatut">
    <img src="https://avatars.githubusercontent.com/bebatut?s=36" alt="Bérénice Batut avatar" width="36" class="avatar" />
  </a>
  
  <a href="/training-material/hall-of-fame/shiltemann/" class="contributor-badge contributor-shiltemann">
    <img src="https://avatars.githubusercontent.com/shiltemann?s=36" alt="Saskia Hiltemann avatar" width="36" class="avatar" />
  </a>
  
  <a href="/training-material/hall-of-fame/lecorguille/" class="contributor-badge contributor-lecorguille">
    <img src="https://avatars.githubusercontent.com/lecorguille?s=36" alt="Gildas Le Corguillé avatar" width="36" class="avatar" />
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