Trim and filter reads - fastp - upgraded

assembly-largegenome/galaxy-workflow-trim-and-filter-reads

Author(s)
Anna Syme
version Version
4
last_modification Last updated
May 7, 2024
license License
GPL-3.0-or-later
galaxy-tags Tags
LG-WF
GTN

Features
Tutorial
hands_on Large genome assembly and polishing
workflow Other workflows associated with this material
Workflow Testing
Tests: ❌
Results: Not yet automated
FAIRness purl PURL
https://gxy.io/GTN:W00016
RO-Crate logo with flask Download Workflow RO-Crate Workflowhub cloud with gears logo View on WorkflowHub
Launch in Tutorial Mode question
galaxy-download Download
flowchart TD
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  style 0 stroke:#2c3143,stroke-width:4px;
  1["ℹ️ Input Dataset\nIllumina reads R2"];
  style 1 stroke:#2c3143,stroke-width:4px;
  2["ℹ️ Input Dataset\nlong reads"];
  style 2 stroke:#2c3143,stroke-width:4px;
  3["fastp on short reads"];
  0 -->|output| 3;
  1 -->|output| 3;
  656e4138-41ab-4561-8989-33de9ac9a2f3["Output\nfastp report on short reads html"];
  3 --> 656e4138-41ab-4561-8989-33de9ac9a2f3;
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  0d53f347-0368-47cb-953a-2e4dac57e013["Output\nfastp filtered R1 reads"];
  3 --> 0d53f347-0368-47cb-953a-2e4dac57e013;
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  10fbe1e5-400c-4ffe-8794-9b776b0d7322["Output\nfastp report on short reads json"];
  3 --> 10fbe1e5-400c-4ffe-8794-9b776b0d7322;
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  639ed3f7-0e51-4e5d-b6f8-081378962109["Output\nfastp filtered R2 reads"];
  3 --> 639ed3f7-0e51-4e5d-b6f8-081378962109;
  style 639ed3f7-0e51-4e5d-b6f8-081378962109 stroke:#2c3143,stroke-width:4px;
  4["fastp on long reads"];
  2 -->|output| 4;
  5e0d2c3d-41a4-4823-ae9c-b1e4d2826541["Output\nfastp report on long reads html"];
  4 --> 5e0d2c3d-41a4-4823-ae9c-b1e4d2826541;
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  e6018ad6-86f4-4e78-8cf2-ccc8b97022fe["Output\nfastp filtered long reads"];
  4 --> e6018ad6-86f4-4e78-8cf2-ccc8b97022fe;
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  69f8383b-a1be-4a74-95f4-3dba35e01426["Output\nfastp report on long reads json"];
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  style 69f8383b-a1be-4a74-95f4-3dba35e01426 stroke:#2c3143,stroke-width:4px;

To use these workflows in Galaxy you can either click the links to download the workflows, or you can right-click and copy the link to the workflow which can be used in the Galaxy form to import workflows.

Importing into Galaxy

Below are the instructions for importing these workflows directly into your Galaxy server of choice to start using them!
Hands-on: Importing a workflow
  • Click on Workflow on the top menu bar of Galaxy. You will see a list of all your workflows.
  • Click on galaxy-upload Import at the top-right of the screen
  • Provide your workflow
    • Option 1: Paste the URL of the workflow into the box labelled “Archived Workflow URL”
    • Option 2: Upload the workflow file in the box labelled “Archived Workflow File”
  • Click the Import workflow button

Below is a short video demonstrating how to import a workflow from GitHub using this procedure:

Video: Importing a workflow from URL

Version History

Version Commit Time Comments
5 1fe24b92f 2024-05-07 09:34:02 Update Galaxy-Workflow-Trim_and_filter_reads.ga
4 e8ddbcdd5 2024-05-01 04:32:16 new versions of workflows
3 12f721099 2024-05-01 04:22:30 Rename Galaxy-Workflow-Trim_and_filter_reads.ga to Galaxy-Workflow-Trim_and_filter_reads_old.ga
2 62e77944b 2023-02-17 09:53:23 add/complete creator info on workflows
1 9fcb3aeec 2022-10-07 06:24:16 large genome tutorial

For Admins

Installing the workflow tools

wget https://training.galaxyproject.org/training-material/topics/assembly/tutorials/largegenome/workflows/Galaxy-Workflow-Trim_and_filter_reads.ga -O workflow.ga
workflow-to-tools -w workflow.ga -o tools.yaml
shed-tools install -g GALAXY -a API_KEY -t tools.yaml
workflow-install -g GALAXY -a API_KEY -w workflow.ga --publish-workflows