Lorum Ipsum

Auteur·rice·s orcid logoSaskia Hiltemann avatar Saskia Hiltemann
Fournisseur·euse·s de données orcid logoHelena Rasche avatar Helena Rasche
Traduction orcid logoHelena Rasche avatar Helena Rasche
Éditeur·rice·s orcid logoSaskia Hiltemann avatar Saskia Hiltemann
Testeur·euse·s orcid logoHelena Rasche avatar Helena Rasche
Réviseur·euse·s Helena Rasche avatar
Expérience utilisateur·rice·s/Designer·euse·s orcid logoSaskia Hiltemann avatar Saskia Hiltemann
Infrastructure orcid logoSaskia Hiltemann avatar Saskia Hiltemann
Description générale
Creative Commons License: CC-BY Questions:
  • Lorem ipsum dolor sit amet,

  • onsectetur adipiscing elit.

Objectifs:
  • Vestibulum sit amet velit nibh

  • Vivamus tincidunt diam ligula, id rutrum purus rutrum a.

Pré-requis:
Durée estimée: 42 Minutes
Niveau: Introduction Introductory
supports:
Publication: Feb 19, 2025
Dernière modification: Feb 19, 2025
Licence: Le contenu de ce didacticiel est sous licence Creative Commons Attribution 4.0 International License. Le Framework GTN est sous licence MIT
version Révision: 1

Test Tutorial for Language: fr

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vestibulum sit amet velit nibh. Nullam porttitor urna at mi vulputate convallis. Cras mattis tempor interdum.

Test all boxes

En pratique: Lorem Ipsum
  1. Lorem Ipsum ..
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Commentaire: Lorem Ipsum
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Question: Lorem Ipsum
  1. Lorem Ipsum ..
  1. Lorem Ipsum ..
  1. Lorem Ipsum ..
Attention: Lorem Ipsum
  1. Lorem Ipsum ..
Entrée: Lorem Ipsum
  1. Lorem Ipsum ..
Sortie: Lorem Ipsum
  1. Lorem Ipsum ..

Test snippets of different box types

To create a new history simply click the new-history icon at the top of the history panel:

UI for creating new history

En pratique: Importing and Launching a Dockstore Workflow
  1. Go to galaxy-workflows-activity Workflows → Import in your Galaxy
  2. Switch tabs to TRS ID
  3. Ensure the “TRS server” is set to “Dockstore”
  4. Provide your “TRS ID” (copied from your workflow’s Dockstore page)
  5. Select the workflow version you want to import

Commentaire: Nanopore sequencing

Nanopore sequencing has several properties that make it well-suited for our purposes

  1. Long-read sequencing technology offers simplified and less ambiguous genome assembly
  2. Long-read sequencing gives the ability to span repetitive genomic regions
  3. Long-read sequencing makes it possible to identify large structural variations

How nanopore sequencing works

When using Oxford Nanopore Technologies (ONT) sequencing, the change in electrical current is measured over the membrane of a flow cell. When nucleotides pass the pores in the flow cell the current change is translated (basecalled) to nucleotides by a basecaller. A schematic overview is given in the picture above.

When sequencing using a MinIT or MinION Mk1C, the basecalling software is present on the devices. With basecalling the electrical signals are translated to bases (A,T,G,C) with a quality score per base. The sequenced DNA strand will be basecalled and this will form one read. Multiple reads will be stored in a fastq file.