Lorum Ipsum
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Descripción GeneralPreguntas:
Objetivos:
Lorem ipsum dolor sit amet,
onsectetur adipiscing elit.
Requisitos:
Vestibulum sit amet velit nibh
Vivamus tincidunt diam ligula, id rutrum purus rutrum a.
Duración estimada: 42 minutosNivel: Introductorio IntroductoryMateriales de apoyo:
Published: Feb 19, 2025Última modificación: Feb 19, 2025Licencia: El contenido de este tutorial tiene la licencia Creative Commons Attribution 4.0 International License. GTN Framework tiene licencia del MIT MITversion Revision: 1
Test Tutorial for Language: es
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vestibulum sit amet velit nibh. Nullam porttitor urna at mi vulputate convallis. Cras mattis tempor interdum.
Test all boxes
Práctica: Lorem Ipsum
- Lorem Ipsum ..
- Lorem Ipsum ..
Comentario: Lorem Ipsum
- Lorem Ipsum ..
Preguntas: Lorem Ipsum
- Lorem Ipsum ..
- Lorem Ipsum ..
- Lorem Ipsum ..
¡Precaucion!: Lorem Ipsum
- Lorem Ipsum ..
Entrada: Lorem Ipsum
- Lorem Ipsum ..
Salida: Lorem Ipsum
- Lorem Ipsum ..
Test snippets of different box types
To create a new history simply click the new-history icon at the top of the history panel:
Práctica: Importing and Launching a Dockstore Workflow
- Go to galaxy-workflows-activity Workflows → Import in your Galaxy
- Switch tabs to TRS ID
- Ensure the “TRS server” is set to “Dockstore”
- Provide your “TRS ID” (copied from your workflow’s Dockstore page)
- Select the workflow version you want to import
Comentario: Nanopore sequencingNanopore sequencing has several properties that make it well-suited for our purposes
- Long-read sequencing technology offers simplified and less ambiguous genome assembly
- Long-read sequencing gives the ability to span repetitive genomic regions
- Long-read sequencing makes it possible to identify large structural variations
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When using Oxford Nanopore Technologies (ONT) sequencing, the change in electrical current is measured over the membrane of a flow cell. When nucleotides pass the pores in the flow cell the current change is translated (basecalled) to nucleotides by a basecaller. A schematic overview is given in the picture above.
When sequencing using a MinIT or MinION Mk1C, the basecalling software is present on the devices. With basecalling the electrical signals are translated to bases (A,T,G,C) with a quality score per base. The sequenced DNA strand will be basecalled and this will form one read. Multiple reads will be stored in a fastq file.