Lorum Ipsum

Autoren orcid logoSaskia Hiltemann avatar Saskia Hiltemann
Datenbereitstellung orcid logoHelena Rasche avatar Helena Rasche
Übersetzer orcid logoHelena Rasche avatar Helena Rasche
Lektoren orcid logoSaskia Hiltemann avatar Saskia Hiltemann
Tester orcid logoHelena Rasche avatar Helena Rasche
Gutachter Helena Rasche avatar
UX/Designer orcid logoSaskia Hiltemann avatar Saskia Hiltemann
Infrastruktur orcid logoSaskia Hiltemann avatar Saskia Hiltemann
Überblick
Creative Commons License: CC-BY Fragen:
  • Lorem ipsum dolor sit amet,

  • onsectetur adipiscing elit.

Lernziele:
  • Vestibulum sit amet velit nibh

  • Vivamus tincidunt diam ligula, id rutrum purus rutrum a.

Voraussetzungen:
Geschätzte Bearbeitungszeit: 42 Minuten
Level: Einsteiger Introductory
Unterstützende Materialien:
Veröffentlicht: Feb 19, 2025
Letzte Änderung: Feb 19, 2025
Lizenz: Der Inhalt des Tutorials ist lizenziert unter der Creative Commons Attribution 4.0 International License. Das GTN Framework ist lizenziert unter MIT
version Überarbeitung: 1

Test Tutorial for Language: de

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vestibulum sit amet velit nibh. Nullam porttitor urna at mi vulputate convallis. Cras mattis tempor interdum.

Test all boxes

Praktische Übung: Lorem Ipsum
  1. Lorem Ipsum ..
  1. Lorem Ipsum ..
Kommentar: Lorem Ipsum
  1. Lorem Ipsum ..
Frage: Lorem Ipsum
  1. Lorem Ipsum ..
  1. Lorem Ipsum ..
  1. Lorem Ipsum ..
Warnung: Lorem Ipsum
  1. Lorem Ipsum ..
Eingabe: Lorem Ipsum
  1. Lorem Ipsum ..
Ausgabe: Lorem Ipsum
  1. Lorem Ipsum ..

Test snippets of different box types

To create a new history simply click the new-history icon at the top of the history panel:

UI for creating new history

Praktische Übung: Importing and Launching a Dockstore Workflow
  1. Go to galaxy-workflows-activity Workflows → Import in your Galaxy
  2. Switch tabs to TRS ID
  3. Ensure the “TRS server” is set to “Dockstore”
  4. Provide your “TRS ID” (copied from your workflow’s Dockstore page)
  5. Select the workflow version you want to import

Kommentar: Nanopore sequencing

Nanopore sequencing has several properties that make it well-suited for our purposes

  1. Long-read sequencing technology offers simplified and less ambiguous genome assembly
  2. Long-read sequencing gives the ability to span repetitive genomic regions
  3. Long-read sequencing makes it possible to identify large structural variations

How nanopore sequencing works

When using Oxford Nanopore Technologies (ONT) sequencing, the change in electrical current is measured over the membrane of a flow cell. When nucleotides pass the pores in the flow cell the current change is translated (basecalled) to nucleotides by a basecaller. A schematic overview is given in the picture above.

When sequencing using a MinIT or MinION Mk1C, the basecalling software is present on the devices. With basecalling the electrical signals are translated to bases (A,T,G,C) with a quality score per base. The sequenced DNA strand will be basecalled and this will form one read. Multiple reads will be stored in a fastq file.