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Submitting SARS-CoV-2 sequences to ENA

Contributors

AvatarMiguel Roncoroni

Objectives

last_modification Last modification: Aug 10, 2021

The European Nucleotide Archive

.pull-left[

.left[ ENA is:

The European Nucleotide Archive and INSDC ]


SARS-CoV-2 sequences

.left[ Why is raw SARS-CoV-2 sequence data important?

.reduce70[Minor allelic-variants can be used to detect intrahost variation. From Maier et al., 2021 doi.org/10.1101/2021.03.25.437046]

Submitting reads with Galaxy

.left[ Why use Galaxy to submit to ENA?


Submission overview

.image-100[ reads-submission. ] —

What you need

.left[ Data:

Metadata:

Credentials:

Metadata

.left[ For the submission of SARS-CoV-2 reads ENA’s metadata model requires:


Metadata

.left[ Interactive metadata input in Galaxy: ] interactive metadata.

Metadata

.left[ Metadata template spreadsheet:

Metadata

.left[

Aliases

.left[ Aliases link metadata objects:

Aliases

.left[ Aliases link metadata objects:

Aliases

.left[ Aliases link metadata to data:


Key Points

Thank you!

This material is the result of a collaborative work. Thanks to the Galaxy Training Network and all the contributors! Galaxy Training Network This material is licensed under the Creative Commons Attribution 4.0 International License.