Workflows

These workflows are associated with Annotating a protein list identified by LC-MS/MS experiments

To use these workflows in Galaxy you can either click the links to download the workflows, or you can right-click and copy the link to the workflow which can be used in the Galaxy form to import workflows.

'Proteome Annotation'

Last updated Jan 22, 2020

Launch in Tutorial Mode question
License: None Specified, defaults to CC-BY-4.0
Tests: ❌ Results: Not yet automated

flowchart TD
  0["ℹ️ Input Dataset\nInput dataset"];
  style 0 stroke:#2c3143,stroke-width:4px;
  1["ℹ️ Input Dataset\nInput dataset"];
  style 1 stroke:#2c3143,stroke-width:4px;
  2["ℹ️ Input Dataset\nInput dataset"];
  style 2 stroke:#2c3143,stroke-width:4px;
  3["Build tissue-specific expression dataset"];
  4["Filter by keywords and/or numerical value"];
  1 -->|output| 4;
  5["ID Converter"];
  3 -->|output| 5;
  6["ID Converter"];
  4 -->|kept_lines| 6;
  7["Venn diagram"];
  4 -->|kept_lines| 7;
  0 -->|output| 7;
  2 -->|output| 7;
  8["Add expression data"];
  6 -->|output| 8;
  9["Filter by keywords and/or numerical value"];
  8 -->|output| 9;
  10["GO terms classification and enrichment analysis"];
  5 -->|output| 10;
  9 -->|kept_lines| 10;
  11["Query pathway database"];
  9 -->|kept_lines| 11;
	
ProteoRE ProteomeAnnotation Tutorial (release 2.0)

Last updated Jan 22, 2020

Launch in Tutorial Mode question
License: None Specified, defaults to CC-BY-4.0
Tests: ❌ Results: Not yet automated

flowchart TD
  0["ℹ️ Input Dataset\nLacombe_et_al_2017.txt"];
  style 0 stroke:#2c3143,stroke-width:4px;
  1["ℹ️ Input Dataset\nBredberg.txt"];
  style 1 stroke:#2c3143,stroke-width:4px;
  10["View proteins on Reactome maps"];
  9 -->|kept_lines| 10;
  11["GO terms classification and enrichment analysis"];
  5 -->|output| 11;
  9 -->|kept_lines| 11;
  2["ℹ️ Input Dataset\nMucilli.txt"];
  style 2 stroke:#2c3143,stroke-width:4px;
  3["Build a lung proteome as a background "];
  4["Filter technical contaminants"];
  0 -->|output| 4;
  5["ID Converter"];
  3 -->|output| 5;
  6["ID mapping UniProt <-> ENSG ID"];
  4 -->|kept_lines| 6;
  7["MS/MS datasets comparison"];
  4 -->|kept_lines| 7;
  1 -->|output| 7;
  2 -->|output| 7;
  8["Add expression data"];
  6 -->|output| 8;
  9["Filter biological contaminants"];
  8 -->|output| 9;
	

Importing into Galaxy

Below are the instructions for importing these workflows directly into your Galaxy server of choice to start using them!
Hands-on: Importing a workflow
  • Click on Workflow on the top menu bar of Galaxy. You will see a list of all your workflows.
  • Click on galaxy-upload Import at the top-right of the screen
  • Provide your workflow
    • Option 1: Paste the URL of the workflow into the box labelled “Archived Workflow URL”
    • Option 2: Upload the workflow file in the box labelled “Archived Workflow File”
  • Click the Import workflow button

Below is a short video demonstrating how to import a workflow from GitHub using this procedure: