MSI Workflow: spatial distribution

Run Workflow in Galaxy

• Galaxy Main
• Galaxy Australia
• Galaxy Europe
• Galaxy France
• Import to another server (≥23.0+ only!)

flowchart TD
  0["ℹ️ Input Dataset\nUploaded Composite Dataset imzml"];
  style 0 stroke:#2c3143,stroke-width:4px;
  1["ℹ️ Input Dataset\nAnnotation"];
  style 1 stroke:#2c3143,stroke-width:4px;
  2["ℹ️ Input Dataset\nm/z features"];
  style 2 stroke:#2c3143,stroke-width:4px;
  3["MSI Qualitycontrol"];
  0 -->|output| 3;
  4["MSI plot spectra"];
  0 -->|output| 4;
  5["MSI filtering"];
  0 -->|output| 5;
  6["MSI plot spectra"];
  0 -->|output| 6;
  1 -->|output| 6;
  7["MSI data exporter"];
  5 -->|outfile_imzml| 7;
  8["MSI mz images"];
  2 -->|output| 8;
  5 -->|outfile_imzml| 8;
  9["Filter"];
  7 -->|feature_output| 9;
  10["MSI mz images"];
  9 -->|out_file1| 10;
  5 -->|outfile_imzml| 10;

Inputs

Outputs

Tools

To use these workflows in Galaxy you can either click the links to download the workflows, or you can right-click and copy the link to the workflow which can be used in the Galaxy form to import workflows.

Importing into Galaxy

Hands-on: Importing a workflow

• Click on Workflow on the top menu bar of Galaxy. You will see a list of all your workflows.
• Click on galaxy-upload Import at the top-right of the screen
• Provide your workflow
  • Option 1: Paste the URL of the workflow into the box labelled “Archived Workflow URL”
  • Option 2: Upload the workflow file in the box labelled “Archived Workflow File”
• Click the Import workflow button

Below is a short video demonstrating how to import a workflow from GitHub using this procedure:

Version History

For Admins

Installing the workflow tools

wget https://training.galaxyproject.org/training-material/topics/metabolomics/tutorials/msi-analyte-distribution/workflows/wf_MSI_distribution.ga -O workflow.ga
workflow-to-tools -w workflow.ga -o tools.yaml
shed-tools install -g GALAXY -a API_KEY -t tools.yaml
workflow-install -g GALAXY -a API_KEY -w workflow.ga --publish-workflows