Identification of the binding sites of the T-cell acute lymphocytic leukemia protein 1 (TAL1)

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  4["ℹ️ Input Dataset\nMegakaryocyte_input_R1_downsampled_SRR492453.fastqsanger"];
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  5["ℹ️ Input Dataset\nMegakaryocyte_input_R2_downsampled_SRR492454.fastqsanger"];
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  7["ℹ️ Input Dataset\nMegakaryocytes_Tal1_R2_downsampled_SRR549007.fastqsanger"];
  style 7 stroke:#2c3143,stroke-width:4px;
  8["ℹ️ Input Dataset\nRefSeq_gene_annotations_mm10.bed"];
  style 8 stroke:#2c3143,stroke-width:4px;
  9["FastQC"];
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  10["Trimmomatic"];
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  30 -->|bam_output| 44;
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  32 -->|bam_output| 45;
  46["plotFingerprint"];
  26 -->|bam_output| 46;
  28 -->|bam_output| 46;
  30 -->|bam_output| 46;
  32 -->|bam_output| 46;
  47["MACS2 callpeak"];
  26 -->|bam_output| 47;
  28 -->|bam_output| 47;
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  34 -->|bam_output| 56;
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  38 -->|bam_output| 56;
  40 -->|bam_output| 56;
  57["bamCompare"];
  40 -->|bam_output| 57;
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  58["computeMatrix"];
  44 -->|outFileName| 58;
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To use these workflows in Galaxy you can either click the links to download the workflows, or you can right-click and copy the link to the workflow which can be used in the Galaxy form to import workflows.

Importing into Galaxy

Hands-on: Importing a workflow

• Click on Workflow on the top menu bar of Galaxy. You will see a list of all your workflows.
• Click on galaxy-upload Import at the top-right of the screen
• Provide your workflow
  • Option 1: Paste the URL of the workflow into the box labelled “Archived Workflow URL”
  • Option 2: Upload the workflow file in the box labelled “Archived Workflow File”
• Click the Import workflow button

Below is a short video demonstrating how to import a workflow from GitHub using this procedure:

Version History

For Admins

Installing the workflow tools

wget https://training.galaxyproject.org/training-material/topics/epigenetics/tutorials/tal1-binding-site-identification/workflows/tal1-binding-site-identification-workflow.ga -O workflow.ga
workflow-to-tools -w workflow.ga -o tools.yaml
shed-tools install -g GALAXY -a API_KEY -t tools.yaml
workflow-install -g GALAXY -a API_KEY -w workflow.ga --publish-workflows