Genome Annotation
Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.
Requirements
Before diving into this topic, we recommend you to have a look at:
Material
Lesson | Slides | Hands-on | Input dataset | Workflows | Galaxy tour |
---|---|---|---|---|---|
Introduction to Genome Annotation
|
slides | ||||
Essential genes detection with Transposon insertion sequencing
|
slides | tutorial Toggle Dropdown | zenodo_link | workflow | |
Genome annotation with Maker
|
tutorial Toggle Dropdown | zenodo_link | workflow | ||
Genome annotation with Prokka
|
slides | tutorial Toggle Dropdown | zenodo_link | workflow | interactive_tour |
Galaxy instances
You can use a public Galaxy instance which has been tested for the availability of the used tools. They are listed along with the tutorials above.
You can also use the following Docker image for these tutorials:
docker run -p 8080:80 quay.io/galaxy/genome-annotation-training
NOTE: Use the -d flag at the end of the command if you want to automatically download all the data-libraries into the container.
It will launch a flavored Galaxy instance available on http://localhost:8080. This instance will contain all the tools and workflows to follow the tutorials in this topic. Login as admin with password password to access everything.
Maintainers
This material is maintained by:
For any question related to this topic and the content, you can contact them or visit our Gitter channel.
Contributors
This material was contributed to by: