Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.

You can view the tutorial materials in different languages by clicking the dropdown icon next to the slides (slides) and tutorial (tutorial) buttons below.


Before diving into this topic, we recommend you to have a look at:


Lesson Slides Hands-on Recordings Input dataset Workflows
Introduction to Genome Annotation
plain text
CRISPR screen analysis plain text tutorial
Essential genes detection with Transposon insertion sequencing plain text tutorial
From small to large-scale genome comparison
plain text tutorial
Functional annotation of protein sequences tutorial
Genome annotation with Funannotate tutorial
Genome annotation with Maker tutorial
Genome annotation with Maker (short) tutorial
Genome annotation with Prokka plain text tutorial
Masking repeats with RepeatMasker tutorial
Refining Genome Annotations with Apollo plain text tutorial

Galaxy instances

You can use a public Galaxy instance which has been tested for the availability of the used tools. They are listed along with the tutorials above.

You can also use the following Docker image for these tutorials:

docker run -p 8080:80

NOTE: Use the -d flag at the end of the command if you want to automatically download all the data-libraries into the container.

It will launch a flavored Galaxy instance available on http://localhost:8080. This instance will contain all the tools and workflows to follow the tutorials in this topic. Login as admin with password password to access everything.

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.


This material is maintained by:

orcid logoAvatarHelena Rasche AvatarSimon Gladman orcid logoAvatarAnthony Bretaudeau

For any question related to this topic and the content, you can contact them or visit our Gitter channel.


This material was contributed to by:

orcid logoAvatarAnthony Bretaudeau orcid logoAvatarHelena Rasche AvatarMaria Doyle AvatarKenji Fujihara AvatarTwishi Gulati AvatarDelphine Lariviere orcid logoAvatarBérénice Batut AvatarEsteban Perez-Wohlfeil orcid logoAvatarAnika Erxleben orcid logoAvatarBjörn Grüning AvatarAnna Syme AvatarTorsten Seemann AvatarSimon Gladman orcid logoAvatarAlexandre Cormier orcid logoAvatarLaura Leroi orcid logoAvatarErwan Corre orcid logoAvatarStéphanie Robin AvatarErasmus+ Programme AvatarNathan Dunn orcid logoAvatarMateo Boudet