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Introduction to CRISPR screen analysis

Contributors

Questions

Objectives

last_modification Last modification: Mar 10, 2022

What is CRISPR?

.center[Overview of CRISPR knockout method]

.image-100[CRISPR method. ]

.pull-right[Adapted from Addgene]

Speaker Notes


What is a CRISPR screen?

.center[CRISPR screens enable high-throughput functional interrogation of a genome]

.image-100[CRISPR screen. ]

Speaker Notes


How are the cells for the screen set up?

.pull-right[

.image-60[sgRNA library. ]

]

.pull-left[

]

Speaker Notes


What is the difference between a negative and positive screen?

.image-100[Neg and pos screens. ]

.pull-right[Adapted from Addgene]

Speaker Notes

A workflow for CRISPR screen analysis

CRISPR workflow.

Speaker Notes


Thank you!

This material is the result of a collaborative work. Thanks to the Galaxy Training Network and all the contributors! Galaxy Training Network This material is licensed under the Creative Commons Attribution 4.0 International License.