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# Reference Data with CVMFS
Updated: Apr 6, 2021
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Useful when presenting. --- ### <i class="fas fa-bullseye" aria-hidden="true"></i><span class="visually-hidden">objectives</span> Objectives - Have an understanding of what CVMFS is and how it works - Install and configure the CVMFS client on a linux machine and mount the Galaxy reference data repository - Configure your Galaxy to use these reference genomes and indices - Use an ansible playbook for all of the above. --- # Built in Data ![List_of_data.png](../../images/i06-List_of_data.png) ??? - Many tools need reference data - E.g. this BWA-MEM tool requires a reference genome - But where do you get this data? - And how do tools know where to find it? --- # Data, what data? .large[ * Some genomes are large! Human, Mouse, Coral * Some tools require indices of the genomes. * The indices take a long time to build! * Better to pre-build the indices. ] ??? - Much of this reference data requires calculation to generate it - For example, building dataset indexes - It is better to build them beforehand, so Galaxy has a concept of reference data --- # Data schematics in Galaxy ![schematic](../../images/data_managers_schematic_overview.png) ??? - Here is a more conrete example - A reference dataset is required as input - A tool (e.g. bwa) is used to build the index - The outputs are registered with Galaxy's data registry in a loc file - The tool data table xml file has a listing of all of the loc files - When a user runs the BWA tool, Galaxy knows where to find this reference data --- # Index Generation with Data Manager - Allows for the **creation of built-in** (reference) data - underlying data - data tables - \*.loc files - Specialized Galaxy tools that can only be accessed by an admin - Defined **locally** or installed from **ToolShed** ??? - Data managers are special tools in Galaxy - They create the reference data - And update the loc files --- # "loc" files - Short for location! ```text # #<unique_build_id> <dbkey> <display_name> <file_path> # bosTau7 bosTau7 Cow (bosTau7) /genomes/bosTau7/bwa_mem_index/bosTau7/bosTau7.fa ce10 ce10 C. elegans (ce10) /genomes/ce10/bwa_mem_index/ce10/ce10.fa danRer7 danRer7 Zebrafish (danRer7) /genomes/danRer7/bwa_mem_index/danRer7/danRer7.fa dm3 dm3 D. melanogaster Apr. 2006 (BDGP R5/dm3) (dm3) /genomes/dm3/bwa_mem_index/dm3/dm3.fa hg19 hg19 Human (hg19) /genomes/hg19/bwa_mem_index/hg19/hg19.fa hg38 hg38 Human (hg38) /genomes/hg38/bwa_mem_index/hg38/hg38.fa mm10 mm10 Mouse (mm10) /genomes/mm10/bwa_mem_index/mm10/mm10.fa ``` ??? - Here is an example loc file - They list all of the indexes of a specific type - Some of these indexes will be tool specific like this one, and some will be more general like a list of genomes - This file is updated by the data manager. --- # Where are the data tables? (Usually located in `galaxy/config/tool_data_table_conf.xml`) ```xml <tables> <!-- Locations of indexes in the BWA mapper format --> <table name="bwa_mem_indexes" comment_char="#" allow_duplicate_entries="False"> <columns>value, dbkey, name, path</columns> <file path="tool-data/bwa_index.loc" /> </table> </tables> ``` ??? - The tool data table conf file lists table names, and their associated loc file - Additionally it defines the meaning of each column in the loc file --- # Using reference data in a tool #### bwa.xml ```xml <param name="ref_file" type="select" label="Using reference genome" help="Select genome from the list"> <options from_data_table="bwa_mem_indexes"> <filter type="sort_by" column="2" /> <validator type="no_options" message="No indexes are available" /> </options> <validator type="no_options" message="A built-in reference genome is not available for the build associated with the selected input file"/> </param> ``` ??? - When a tool wants to use the data from those tables, it needs to declare which tables it wants to access (see previous slide) - A select type parameter is created for the user's selections - The tool knows that the options should come from a BWA-MEM indexes loc file - These reference datasets are sorted by column 2, the name - An some validators ensure a helpful message is shown if no data is available --- # Some Problems! - Super complex, right? - Time consuming! - ~30 minutes work just to add a new genome to 1 tool! - Administrator needs to know: - how to index **every** tool - expected format of the reference data - format of the .loc file - Some parts solved by Data Managers - **But there's an easier way!** ??? - This was a lot of information - And very genomics specific in some places - A lot of work to create and update the reference data - But there is a better way! --- # There's a lot of reference data .large[ (and it's hard to keep up with) ] ![ref_data_prob_flow.png](../../images/ref_data_prob_flow.png) ??? - Imagine going through this process every time a user request comes in - It would be unpleasant --- # CernVM-FS to the rescue - Needed a method of sharing reference data across country efficiently - **CVMFS** is an efficient method for read only data sharing between systems - Originally designed for distributed software installation at Cern - Turns out it's really useful for read only data sets as well - HTTP-based, firewall friendly - All nodes of Galaxy Main get their reference genomes and indices from CVMFS - Shared via mirroring and caching across the country - It's also really useful to share data **globally** - The **usegalaxy.\*** initiative has taken full advantage of this. ??? - CVMFS and the IDC solves these issues - CVMFS provides a global repository of reference data - Originally built by CERN for sharing software, we use it for data - It's an HTTP based protocol and very firewall friendly - All of the usegalaxy.* servers host a CVMFS repository --- # IDC - Group of admins working on the CVMFS data repository - Wish to automate the management of data and generation of new indicies - Come join us: [github.com/galaxyproject/idc](https://github.com/galaxyproject/idc) ??? - The IDC is the other half of the puzzle - CVMFS provides the storage, and the IDC provides the data - Join us on github if you are interested --- # CVMFS Global Structure .widen_image[ ![cvmfs_global_structure.png](../../images/cvmfs_global_structure.png) ] ??? - CVMFS has a hierarchical structure - At the top is the stratum 0 server, the original copy of the datasets - This is replicated to the read-only stratum 1 servers - Anyone can connect to these stratum 1 servers - And whenever connections fail, CVMFS will fail over to another mirror - The mirror selection process is based on connection round trip times - As a result, the nearest mirror is usually selected. --- .widen_image[ ![cvmfs_server_distribution.png](../../images/cvmfs_server_distribution.png) ] ??? - There are CVMFS servers across the entire world - The primary mirrors are run by Galaxy project, Galaxy Europe, and Galaxy Australia - If one of these mirrors fails, you will still be able to use the reference data --- ## Thank You! This material is the result of a collaborative work. Thanks to the [Galaxy Training Network](https://training.galaxyproject.org) and all the contributors!
This material is licensed under the Creative Commons Attribution 4.0 International License