Genome Annotation

Genome annotation is a multi-level process that includes prediction of protein-coding genes, as well as other functional genome units such as structural RNAs, tRNAs, small RNAs, pseudogenes, control regions, direct and inverted repeats, insertion sequences, transposons and other mobile elements.


Before diving into this topic, we recommend you to have a look at:


Lesson Slides Hands-on Input dataset Workflows Galaxy tour
Introduction to Genome Annotation
level level level
Essential genes detection with Transposon insertion sequencing
slides tutorial zenodo_link workflow
From small to large-scale genome comparison
slides tutorial zenodo_link workflow
Genome annotation with Maker
level level level
tutorial zenodo_link workflow
Genome annotation with Maker (short)
level level level
tutorial zenodo_link workflow
Genome annotation with Prokka
level level level
slides / video tutorial zenodo_link workflow interactive_tour

Galaxy instances

You can use a public Galaxy instance which has been tested for the availability of the used tools. They are listed along with the tutorials above.

You can also use the following Docker image for these tutorials:

docker run -p 8080:80

NOTE: Use the -d flag at the end of the command if you want to automatically download all the data-libraries into the container.

It will launch a flavored Galaxy instance available on http://localhost:8080. This instance will contain all the tools and workflows to follow the tutorials in this topic. Login as admin with password password to access everything.

Frequently Asked Questions

Common questions regarding this topic have been collected on a dedicated FAQ page . Common questions related to specific tutorials can be accessed from the tutorials themselves.


This material is maintained by:

Helena RascheHelena Rasche Simon GladmanSimon Gladman Anthony BretaudeauAnthony Bretaudeau

For any question related to this topic and the content, you can contact them or visit our Gitter channel.


This material was contributed to by:

Anthony BretaudeauAnthony Bretaudeau Helena RascheHelena Rasche Delphine LariviereDelphine Lariviere Bérénice BatutBérénice Batut Esteban Perez-WohlfeilEsteban Perez-Wohlfeil Anna SymeAnna Syme Torsten SeemannTorsten Seemann Simon GladmanSimon Gladman