Metagenomics
Metagenomics is a discipline that enables the genomic study of uncultured microorganisms
Requirements
Before diving into this topic, we recommend you to have a look at:
Material
Lesson | Slides | Hands-on | Input dataset | Workflows | Galaxy tour |
---|---|---|---|---|---|
Introduction to metagenomics
|
slides | ||||
16S Microbial Analysis with mothur (extended)
|
tutorial Toggle Dropdown | zenodo_link | workflow | interactive_tour | |
16S Microbial Analysis with mothur (short)
|
tutorial Toggle Dropdown | zenodo_link | workflow | ||
Analyses of metagenomics data - The global picture
|
tutorial Toggle Dropdown | zenodo_link | workflow | interactive_tour | |
Antibiotic resistance detection
nanopore
plasmids
|
tutorial Toggle Dropdown | zenodo_link | workflow | interactive_tour | |
Metatranscriptomics analysis using microbiome RNA-seq data
level level level
metatranscriptomics
|
tutorial Toggle Dropdown | zenodo_link | workflow |
Galaxy instances
You can use a public Galaxy instance which has been tested for the availability of the used tools. They are listed along with the tutorials above.
You can also use the following Docker image for these tutorials:
docker run -p 8080:80 quay.io/galaxy/metagenomics-training
NOTE: Use the -d flag at the end of the command if you want to automatically download all the data-libraries into the container.
It will launch a flavored Galaxy instance available on http://localhost:8080. This instance will contain all the tools and workflows to follow the tutorials in this topic. Login as admin with password admin to access everything.
Maintainers
This material is maintained by:
For any question related to this topic and the content, you can contact them or visit our Gitter channel.
Contributors
This material was contributed to by:
References
- Kozich JJ, Westcott SL, Baxter NT, Highlander SK, Schloss PD.: Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform. Applied and Environmental Microbiology
- Pablo Yarza, Pelin Yilmaz, Elmar Pruesse, Frank Oliver Glöckner, Wolfgang Ludwig, Karl-Heinz Schleifer, William B. Whitman, Jean Euzéby, Rudolf Amann, Ramon Rosselló-Móra: Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences.