Assembly

DNA sequence data has become an indispensable tool for Molecular Biology & Evolutionary Biology. Study in these fields now require a genome sequence to work from. We call this a 'Reference Sequence.' We need to build a reference for each species. We do this by Genome Assembly. De novo Genome Assembly is the process of reconstructing the original DNA sequence from the fragment reads alone.

Requirements

Before diving into this topic, we recommend you to have a look at:

Material

Lesson Slides Hands-on Input dataset Workflows Galaxy tour
De Bruijn Graph Assembly
Introduction to Genome Assembly
Making sense of a newly assembled genome
prokaryote
Unicycler Assembly
prokaryote

Galaxy instances

You can use a public Galaxy instance which has been tested for the availability of the used tools. They are listed along with the tutorials above.

Maintainers

This material is maintained by:

Simon GladmanSimon Gladman, Anton NekrutenkoAnton Nekrutenko, Delphine LariviereDelphine Lariviere

For any question related to this topic and the content, you can contact them or visit our Gitter channel.

Contributors

This material was contributed to by:

Simon GladmanSimon Gladman, Helena RascheHelena Rasche, Saskia HiltemannSaskia Hiltemann, Anton NekrutenkoAnton Nekrutenko, Delphine LariviereDelphine Lariviere